IRB Company Essay Example | Topics and Well Written Essays - 500 words

IRB Company - Essay Example Data Gathering Instrument(s): Not addressed from the previous review. Please include all data gathering instruments in the portfolio. IRB needs to review these. I have already included the interview guide. Let me know if you still do not have it so I upload it to you again. How will the participants be contacted about the study?Three levels of contact shall be designed. The first shall be through the use of official e-mail where the researcher shall contact the General Managers of these companies. Upon receiving a reply from the companies, the researcher shall then follow up with a telephone call to all the 8 companies. Where applicable, the researcher shall contact the companies in person to introduce the purpose of the research and the role to be played by the respondents to the managers. Who will contact the potential participants and in what manner? (in person, via email, via the phone, etc.) Also, if contact will be made at a place of business, via a business email or phone numb er, permission from that business too.Potential participants shall be human resource managers. These will first be contacted through their General Managers, using the official e-mail of the companies. Once the researcher visits the companies in person, the human resource managers shall then be contacted in person and presented with the consent forms, which will contain the purpose of the study, the role of participants, and arrangements made to secure the anonymity, confidentiality, and safety of participants.

Enzymes activity Lab Report Example | Topics and Well Written Essays - 1750 words

Enzymes activity - Lab Report Example The three sets of reactions were prepared by adding varying amounts of 0.25M sucrose and distilled water. In one set, 0.2 ml of 75mM of magnesium chloride was added, and in another set, an equal amount of 4M Urea was added as inhibitors to the reaction. Reactions in living organisms are slow, enzymes speed up the reactions so as to maintain life. The enzymes control the reactions by ensuring that every reaction is catalyzed by a specific enzyme and at a particular point in a cell. They have a tertiary structure and are folded in a conformation that many intramolecular interactions of amino acids that make up the molecule. They are not used up in the reactions therefore can be used in several reactions. They are substrate specific that is they fold in a shape assisted by chaperone proteins that will determine which substrate the enzyme will act upon. Coenzymes and cofactors aid enzymes in their functions. Enzymes can be denatured by extremes in temperature and pH. Competitive inhibitors, noncompetitive inhibitors and allosteric inhibitors regulate enzyme activity. The enzymes do not change the reactions’ equilibrium since the free energies of the reactants or products are not changed. Enzymes are catalysts that are protein in nature that increase the rate of a chemical reaction by providing an alternative reaction pathway with a lower free energy of activation. The reaction is usually expressed as In enzyme kinetics, the Michaelis Menten reaction relates the reaction rate (ÃŽ ½) to [S] which is the concentration of a substrate. The Km and the Vmax of an enzyme can be determined from determination of the initial rates of the enzyme catalyzed reactions over the concentration of the substrate. The maximum rate achieved is Vmax while Km is the Michaelis constant, is the substrate concentration at which the reaction rate is half of Vmax. Km varies from one enzyme to another but is